An R2R3-MYB Transcription Factor RoMYB10 Regulates Anthocyanin Biosynthesis in Black Raspberry

At present, there are many excellent cultivars of raspberry worldwide with different fruit colors, such as yellow, red, purple and black. Anthocyanin accumulation is responsible for flower coloration in plants. Anthocyanins belong to flavonoids which natural pigments widely distributed The MYB transcription factor (TF) plays a vital regulatory role the metabolic pathway anthocyanins. Here, RoMYB10, an R2R3-MYB black (Rubus occidentalis L.), was isolated. basic physical chemical properties, conserved motif distribution, evolutionary relationship subcellular localization were analyzed RoMYB10 encoded amino acid sequence. contents anthocyanin determined four colored fruits by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS). expression levels MYB10 genes various tissues at developmental stages raspberries detected real-time fluorescence quantitative PCR (qRT-PCR). results showed that gene highest raspberry. Moreover, significantly higher than other tissues, especially ripe mottled fruits. When ectopic overexpression occurred tobacco, total transgenic tobacco plants those wild-type (WT) In addition, up-regulated key biosynthesis, NtCHS, NtCHI, NtF3H, NtANS NtUFGT. Our study suggests involved regulating biosynthesis has potential molecular tool manipulating anthocyanins using engineering.